Integral System Yeasts Plus

INTEGRAL SYSTEM YEASTS Plus (ref. 71822) is a 24 wells system containing biochemical substrata and dried antimycotics for the identification of the most clinically important yeasts and sensitivity evaluation to antimycotics. The system is inoculated with the cell suspension and incubated at 36 °C for 48 hours.

PRINCIPLE OF THE METHOD:

  •  Presumptive Identification.
It is based on assimilation reactions of sugars; the tests for the assimilation  reactions are interpreted by evaluating the color change of wells 1-GLU to 12-DUL. The combination of positive and negative reactions allows the formation of a numerical code which permits to identify the yeasts under examination.

  •  Sensitivity to antimycotics.
The tests are evaluated according to growth or inhibition of yeasts in media containing the antimycotic and a growth indicator, in the wells 13-NY to 23-FLU.
The color change from red to orange in the wells indicates a slow growth of the yeast under examination and an intermediate sensitivity to the concentration of antimycotic in the well.
The color change from red to yellow in the wells indicates a growth of the yeast under examination and resistance to the concentration of antimycotic in the well.
No color change in the well indicates no growth of the yeast under examination and sensitivity to the concentration of antimycotic in the well.
The well 24-C does not contain antimycotics, it contains culture medium and indicator and it works as growth control.

View the INTEGRAL SYSTEM YEASTS PLUS in the e-catalogue

References
1. Murray, Baron, Pfaller, Tenorev and Yolken. Manual of Clinical Microbiology 8th Edition (2003)
2. Bayley and Scott’s. Diagnostic Microbiology 7thEdition (1986).
3. Davise H. Larone. “Medically important fungi: a guide to identification”. 2ThEdition. Elsevier (1987).
4. J.P. Van der Walt, L.Rodrigues De Miranda, S.A.Meyer. “The Yeasts ”. 3ThEdition. Elsevier (1984).
5. Barchiesi, F., A.M. Tortorano, l.F. Di Francesco, M. Cogliati, G.scalise, and M.A.Viviani.
In vitro activity of five antifungal agents against uncommon clinical isolates of Candida spp. J.Antimicrob.Chemother. 43.295-299 (1999).
6. NCCLS. Reference method for broth dilution antifungal susceptibility testing of yeasts. M27-A2.(2002).